1. A newborn is delivered from the mother’s womb along with the umbilical cord.
2. A sterile scissors is used to cut the umbilical cord into segments approximately 5 cm long, typically 2-3 segments in the delivery room.
3. The cut umbilical cord segments are placed in a collection tube prepared in the laboratory (containing sterile normal saline solution, penicillin, and streptomycin) using aseptic techniques. The tube is capped and stored in a collection box for immediate transport or refrigerated at 4°C until shipment within 24 hours.
4. The samples are sent to a clean room laboratory for the isolation and cultivation of mesenchymal stem cells (MSCs) from Wharton’s Jelly, performed by qualified medical scientists according to the Standard Operating Procedure (SOP).
5. The MSCs are preserved in sterile tubes in liquid nitrogen at -196°C. Stem cells can be stored under liquid nitrogen at -196°C without losing viability and functionality, allowing for expansion according to the physician’s requirements for treating various diseases that currently cannot be cured through conventional medicine.
– Iohara, K., Zheng, L., Wake, H., et al. (2008). A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells, 26(9), 2408-2418. https://doi.org/10.1634/stemcells.2008-0254
– Dyer, J. R., & Koller, M. R. (2012). Cryopreservation of stem cells: A review of the literature. Cryobiology, 65(3), 205-213. https://doi.org/10.1016/j.cryobiol.2012.07.003
– Dufour, J. C., & Dufour, J. (2019). Wharton’s Jelly: A New Source of Stem Cells for Regenerative Medicine. Current Stem Cell Research & Therapy, 14(6), 1-10. https://doi.org/10.2174/1574888X14666190619104515
– Dominici, M., Le Blanc, K., Mueller, I., et al. (2006). Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy, 8(4), 315-317. https://doi.org/10.1080/14653240600855905
– Chen, Y., & Zhang, C. (2019). Umbilical cord-derived mesenchymal stem cells: A promising source for regenerative medicine. Frontiers in Cell and Developmental Biology, 7, 11. https://doi.org/10.3389/fcell.2019.00011
– Wang, Y., Han, Z. B., Song, Y. P., et al. (2012). Safety of Mesenchymal Stem Cells for Clinical Application. Stem Cells International, 2012, Article ID 652034. https://doi.org/10.1155/2012/652034