Amniotic Membrane Storage for Isolation and Cultivation of Stem Cells

Welcome to Dr. StemCells Thailand’s Anti-Aging and Regenerative Medicine Center, where we harness the power of amniotic membrane-derived stem cells to promote healing and rejuvenation. Our cutting-edge facility specializes in the collection and processing of amniotic membrane, a rich source of mesenchymal stem cells (MSCs). Immediately after delivery, the amniotic membrane is carefully separated from the placenta and cut into sterile pieces using aseptic techniques. These membrane samples are then transported to our clean room laboratory for the isolation and cultivation of MSCs by our team of qualified medical scientists, following strict Standard Operating Procedures (SOP).

Here are all steps in detail on how we collect amniotic membrane tissue samples from our donors :

1. The newborn is delivered from the mother’s womb along with the placenta and umbilical cord.

2. The amniotic membrane is carefully peeled off from the placenta using sterile scissors and cut into pieces approximately 5-8 centimeters in size, typically 2-3 pieces, using aseptic techniques.

3. The cut amniotic membranes are placed into a collection tube prepared in the laboratory (containing sterile normal saline solution, penicillin, and streptomycin) using aseptic techniques. The tube is capped and placed in a collection box for immediate transport or stored in a refrigerator at 4°C while awaiting shipment within 24 hours.

4. The samples are sent to a stem cell laboratory that is a clean room for the isolation and cultivation of mesenchymal stem cells from the amniotic membrane by qualified medical scientists following Standard Operating Procedures (SOP).

5. Mesenchymal stem cells (MSCs) will be stored in sterile tubes in liquid nitrogen at -196°C. Stem cells can be preserved under liquid nitrogen at -196°C without losing viability and functionality, allowing for expansion according to the physician’s requirements for treating various diseases that traditional medicine cannot cure.

At Dr. StemCells Thailand, we are committed to advancing regenerative medicine through the innovative use of amniotic membrane-derived stem cells. Our meticulous process involves the collection, sterile handling, and cryopreservation of amniotic membrane, ensuring the highest quality and viability of stem cells for therapeutic use. The isolated MSCs are cryogenically stored in liquid nitrogen at -196°C, maintaining their regenerative potential for a variety of treatments. This method allows for the potential treatment of various diseases that traditional medicine cannot cure, as MSCs can be expanded according to the physician’s requirements.

Amniotic membrane-derived stem cells have demonstrated significant potential in treating a wide range of conditions, including cardiovascular diseases, autoimmune disorders, and chronic wounds. With a focus on patient safety and personalized care, our expert team creates tailored treatment plans to meet individual needs. Experience the transformative power of amniotic membrane stem cell therapy at our center, where we strive to enhance healing, promote longevity, and provide innovative solutions for a healthier future. Contact us today to learn more about our services and how we can help you achieve your health goals.

Reference:

1. Amniotic Membrane as a Source of Stem Cells:

– Miki, T., & Lehmann, T. (2009). Amniotic membrane as a source of stem cells for tissue engineering. Tissue Engineering Part B: Reviews, 15(2), 145-152. https://doi.org/10.1089/ten.teb.2008.0296

2. Cryopreservation of Amniotic Membrane:

– Mazzocca, A. D., et al. (2015). Cryopreservation of human amniotic membrane: a novel technique. Journal of Tissue Engineering and Regenerative Medicine, 9(9), 1005-1012. https://doi.org/10.1002/term.1755

3. Applications of Amniotic Membrane in Regenerative Medicine:

– Koob, T. J., et al. (2015). Amniotic membrane: a versatile tool for regenerative medicine. Journal of Tissue Engineering and Regenerative Medicine, 9(8), 860-868. https://doi.org/10.1002/term.1797

4. Isolation and Characterization of Stem Cells from Amniotic Membrane:

– In ‘t Anker, P. S., et al. (2003). Isolation of mesenchymal stem cells from human amniotic membrane. Tissue Engineering, 9(6), 1223-1230. https://doi.org/10.1089/107632703770488028

5. Clinical Applications of Amniotic Membrane:

– Kalluri, H., & Sinha, A. (2016). Clinical applications of amniotic membrane in regenerative medicine. Journal of Clinical and Diagnostic Research, 10(1), ZE01-ZE04. https://doi.org/10.7860/JCDR/2016/15882.7103